Journal: The Journal of Biological Chemistry

Article Title: Genetic Screen Reveals Link between the Maternal Effect Sterile Gene mes-1 and Pseudomonas aeruginosa- induced Neurodegeneration in Caenorhabditis elegans *

doi: 10.1074/jbc.M115.674259

Figure Lengend Snippet: Neurodegeneration phenotypes induced by P. aeruginosa infection. A, summary of the morphological changes observed in animals infected with P. aeruginosa. B, images of ASE neurons of strain OH4168 and the ASER neuron of strain OH3192 exposed to control E. coli or P. aeruginosa. C, images of the AWC neuron of strain CX5974 exposed to control E. coli or P. aeruginosa. Different morphological changes in the neurons are indicated by white arrows. A–C, synchronized L4 nematodes were cultured on plates containing full lawns of P. aeruginosa for 30 h at 25 °C and then visualized using a Leica M165 FC stereomicroscope. Scale bars, 50 μm. D, chemotaxis of L4 nematodes exposed to E. coli or P. aeruginosa. Asterisks indicate statistical significance (**, p < 0.01). The data represent the results of three independent experiments. Bars, mean ± S.E. (error bars).

Article Snippet: The animals were mounted onto agar pads and visualized using a Zeiss Axioscope microscope or Leica M165 FC microscope.

Techniques: Infection, Cell Culture, Chemotaxis Assay

Journal: The Journal of Biological Chemistry

Article Title: Genetic Screen Reveals Link between the Maternal Effect Sterile Gene mes-1 and Pseudomonas aeruginosa- induced Neurodegeneration in Caenorhabditis elegans *

doi: 10.1074/jbc.M115.674259

Figure Lengend Snippet: Effect of osmotic stress on survival and neurodegeneration. A, survival curves of OH3192 young adults grown on standard 50 mm NaCl or increasing concentrations of NaCl. B, neurodegeneration rates in OH3192 young adults cultured on plates containing 50 mm NaCl or increasing concentrations of NaCl for 30 h at 25 °C followed by visualization using a Leica M165 FC stereomicroscope. Asterisks indicate significant differences (**, p < 0.01; NS, not significant). The data represent the results of three independent experiments (n = 60 ± 5 animals). Bars, mean ± S.E. (error bars).

Article Snippet: The animals were mounted onto agar pads and visualized using a Zeiss Axioscope microscope or Leica M165 FC microscope.

Techniques: Cell Culture

Journal: The Journal of Biological Chemistry

Article Title: Genetic Screen Reveals Link between the Maternal Effect Sterile Gene mes-1 and Pseudomonas aeruginosa- induced Neurodegeneration in Caenorhabditis elegans *

doi: 10.1074/jbc.M115.674259

Figure Lengend Snippet: ac319 sterile animals are resistant to P. aeruginosa infection and P. aeruginosa-induced neurodegeneration. A, survival curves of OH3192, ac319 fertile, and ac319 sterile young adults exposed to P. aeruginosa. p < 0.0001, OH3192 versus ac319 sterile animals. B, images of the ASER neuron of OH3192, ac319 fertile, and ac319 sterile young adults exposed to P. aeruginosa. Scale bar, 50 μm. C, neurodegeneration rates of OH3192, ac319 fertile, and ac319 sterile young animals exposed to P. aeruginosa. Synchronized young adult animals were cultured on full lawn plates of P. aeruginosa for 30 h at 25 °C. The neurodegeneration rate of OH3192, ac319 fertile, and ac319 sterile animals was scored using a Leica M165 FC stereomicroscope. Asterisks indicate significant differences (*, p < 0.05; **, p < 0.01). The graph represents the combined results of three independent experiments (n = 60 ± 5 animals). Bars, mean ± S.E. (error bars).

Article Snippet: The animals were mounted onto agar pads and visualized using a Zeiss Axioscope microscope or Leica M165 FC microscope.

Techniques: Infection, Cell Culture